The surface of pure titanium is usually modified in order to make it bioactive due to its thin and non-bioactive oxide film generated in air. In this paper, in order to improve the bioactivity of titanium implants, titanium dioxide (0102) nanotube layer was prepared on the surface of titanium by electrochemical anodic oxidation. Then the bioactivity of the "1"102 nanotube layer was further enhanced by biological activation method, and the bioactivity and biocompatibility of the modified surface were studied by simulated body fluid (sBF) immersion and osteoblast culture experiments. Ti02 nanotubes prepared on the surface of titanium were continuously distributed, closely arranged, and grew perpendicular to the surface of titanium. The formation of nanotube layer is influenced by parameters such as voltage, concentration of hydrofluoric acid (m-port), anodic oxidation time and pH value. In a certain voltage range, surface 02 nanotubes with different diameters can be obtained at different voltages. I - IF the concentration can decide the size of the nanotube structure formation, too big or too small can't get Ⅲ 02 nanotubes on the titanium surface layer; Time is a key factor in determining the length of nanotubes. X-ray diffraction (XRD) analysis showed that the oxide film obtained in the anodic oxidation process was amorphous Ti02, and anatase titanium dioxide was obtained after heat treatment, and SEM analysis showed that the heat treatment did not destroy the structure of field D2 nanotube layer. The contact Angle test results show that ti with Ti02 nanotube layer has higher surface energy. The ah 02 nanotube layer was treated with pre-calcification and low concentration alkali, and then mineralized to deposit hydroxyapatite (HA).
Compared with untreated samples, HA is more likely to deposit in the Ti02 nanotube layer. This may be because the calcium carbonate crystals formed on the surface of the pre-calcified samples were dissolved in the immersion process, so that the local Ca and P ion concentrations on the surface increased, and HA began to nucleate and grow on the surface. Hydroxyl groups appeared on the surface of the samples after low-concentration alkali treatment, and the presence of hydroxyl groups changed the element and charge distribution on the surface of the samples. In this state, HA was more likely to deposit on the surface. On the other hand, samples without activation treatment were immersed in high ion concentration Sm. HA can be deposited on the n02 nanotube layer in a short period of time due to increased supresaturation (3 SBF). The binding strength between the HA coating and the w02 nanotube layer was greater than 15. 3 mpa. Titanium surface nano structured high southwest jiaotong university graduate dissertation first page Ⅱ surface energy, and can provide more HA nucleation points, which may lead to coating and currency 02 nanotube layer has a high bonding strength. Osteoblast culture was performed on titanium samples, titanium samples with 'n02 nanotube layer and HA coated samples. The results showed that the cells were more likely to adhere to the titanium-like surface with surface 02 nanotube layer and had higher differentiation ability. The surface containing ca and P elements can also promote cell adhesion and differentiation. The surface energy and element distribution of the samples may be the main factors affecting cell adsorption, proliferation and differentiation. This indicates that the prepared oxide film and i-ia coating have better biocompatibility.
